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4A Biotech
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Meridian Life Science
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ImmunoTools
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Image Search Results
Journal: Tissue engineering
Article Title: Improved hepatocyte excretory function by immediate presentation of polarity cues.
doi: 10.1089/ten.2006.12.2181
Figure Lengend Snippet: FIG. 1. Quantification of the spread of ZO-1 from the cell boundary. The gray component of the images of CD147-stained cells (A) was thresholded and subjected to a thinning algorithm to give 1-pixel-thick cell boundaries, image I1 (C). The green component of the ZO-1-stained cells (B) was thresholded to give image I2 (D). (E) Distance map of ZO-1 from cell boundary and the gray levels indicate distance from cell boundary. Less spread of ZO-1 from the cell boundary implies better tight-junctions formation. Color images available online at www.liebertpub.com/ten.
Article Snippet:
Techniques: Staining
Journal: Tissue engineering
Article Title: Improved hepatocyte excretory function by immediate presentation of polarity cues.
doi: 10.1089/ten.2006.12.2181
Figure Lengend Snippet: FIG. 3. Kinetics of formation of apical and basolateral domains by double-staining for apical multidrug resistance–associated protein (Mrp2) (red) and basolateral CD147 (green). (A) Immediate overlay. (B) 24-h overlay with insert. (C) Conventional 24-h overlay. Arrows point to Mrp2 concentrated in localized regions of cell-cell contacts. Color images available online at www.liebertpub.com/ten.
Article Snippet:
Techniques: Double Staining
Journal: Tissue engineering
Article Title: Improved hepatocyte excretory function by immediate presentation of polarity cues.
doi: 10.1089/ten.2006.12.2181
Figure Lengend Snippet: FIG. 5. Formation of tight junctions at 48 h of culture. (A–C) XYZ reconstruction of ZO-1 (green) counterstained with basolateral CD147 (gray). (A) Immediate overlay. (B) 24-h overlay with insert. (C) Conventional 24-h overlay. (D) Transmission electron micrograph of the cell boundary hepatocytes in immediate overlay cultured for 48 h. Typical microvilli in the biliary canaliculi (BC) and tight junctions on the surrounding plasma membrane (arrowheads) can be observed. Scale bar refers to 0.5 mm. Color images available online at www.liebertpub.com/ten.
Article Snippet:
Techniques: Transmission Assay, Cell Culture, Clinical Proteomics, Membrane